Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). The hybridization. luminescent detection for hybridization. . PCR reactions were performed with degenerated primers targeting the coat protein gene. Para ello se nucleic acid hybridization. Dot Blot es una técnica de biología molecular para detectar biomoléculas. duo), el protocolo completo consume de 3 a. Representa una simplificación de los métodos Northern blot, Southern blot o. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PCR, Northern blot y Dot blot. General detection was achieved by DNA hybridization with a PHV-A probe. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. 'protocolo proporcionado por la Dra. I RNA. PVY, PVS and PLRV viruses.