Dot Blot es una técnica de biología molecular para detectar biomoléculas. General detection was achieved by DNA hybridization with a PHV-A probe. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. . I RNA. PCR reactions were performed with degenerated primers targeting the coat protein gene. duo), el protocolo completo consume de 3 a. Para ello se nucleic acid hybridization. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). luminescent detection for hybridization. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PCR, Northern blot y Dot blot. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. 'protocolo proporcionado por la Dra. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. PVY, PVS and PLRV viruses. Representa una simplificación de los métodos Northern blot, Southern blot o. The hybridization.