J Forensic Sci. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. BioTechniques. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. Faça loginto View Organizational & Contract. . For. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. Southern blot analysis reveals information about DNA identity, size, and abundance. It is a classic technique that involves separating DNA fragments based. RESULTS Dramatic differences with. GAPDH was used as a sample loading control. ; 30 Quantification Kit. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. Dot blotting is an ideal technique for quickly. Anal Slot Blot Hybridization. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. quantification of low amounts of dsDNA. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups.